Application | Comment | Organism |
---|---|---|
pharmacology | tropane alkaloids including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, improvement of production by metabolic engineering introduction of genes encoding the branch-controlling enzyme tropinone reductase I and the downstream rate-limiting enzyme hyoscyamine-6beta-hydroxylase | Anisodus acutangulus |
Cloned (Comment) | Organism |
---|---|
overexpression of the enzyme in hairy roots of transgenic Anisodus acutangulus using Agrobacterium tumefaciens strain C58C1 transfection method, coexpression with tropinone reductase I | Anisodus acutangulus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enhancied production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropanalkaloid contents in transgenic lines, overview | Anisodus acutangulus |
additional information | enhancied production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase, quantification of tropan alkaloid contents in transgenic lines, overview | Anisodus acutangulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
scopolamine + succinate + CO2 | - |
? | |
(6S)-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
scopolamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anisodus acutangulus | - |
- |
- |
Anisodus acutangulus | A2IBI0 | cf. EC 1.14.11.11 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hairy root | - |
Anisodus acutangulus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | scopolamine + succinate + CO2 | - |
? | |
(6S)-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | scopolamine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H&H | - |
Anisodus acutangulus |
H6H | - |
Anisodus acutangulus |
hyoscyamine-6beta-hydroxylase | - |
Anisodus acutangulus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in biosynthesis pathway of tropane alkaloids catalyzing two oxidation steps to form scopolamine from hyoscyamine, overview, cf. EC 1.14.11.11 | Anisodus acutangulus |